EFFECT OF UNIVERSITY OF WISCONSIN SOLUTION ON ISCHEMIA-REPERFUSION DAMAGE IN RENAL PRESERVATION

The preservation solution in cadaveric renal transplantation has been shown to be one of the major factors influencing the incidence of postoperative allograft dysfunction. The purpose of our study was to examine the ultrastructural and biochemical adverse effects of cold ischemia in the renal preservation with simple cold storage, and to investigate the efficacy of University ofWiscosin (UW) solution in the prevention of these adverse effects. 

Rabbits in the first group underwent nephrectomy and perfused with the Euro-Collins (EC) solution, after 48 and 96 hours reperfusion was done with the blood of the same animal. Perfusion and preservation in the second group was done with the UW solution. The samples from the kidney were examined under electron-microscopy (EM) and lipid peroxidation and glutation levels were analysed. In the EM examination of the first group; red blood cell (RBC) trapping, erythrocyt agglutation and endothelium adhesion were the major findings after 24 h duration of preservation. Endothelial defects and erythrocyte extravasation were observed in the kidneys underwent reperfusion. Although RBC trappingwas seen in the second group, agglutination and adhesionwas not seen in the 24 h preservation time. Mitochondria of the tubulus and microvilli were better protected and the erythrocyte extravasation was less prominent.

We concluded that UW solution causes less damage to the mitochondria and microvilli by maintaining hight glutation levels; prevents the adhesion of erythrocytes to the endothelium membrane during 24 h preservation; thereby lessening the damage caused by ischemic reperfusion.