Turkish Journal of Nephrology
Original Article

Hypericum perforatum Extract Increased Necrosis in Amikacin-Induced Kidney Injury

1.

Department of Nephrology, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey

2.

Department of Biochemistry, Faculty of Pharmacy, Karadeniz Technical University, Trabzon, TURKEY

3.

Department of Histology and Embryology, Faculty of Medicine, Karadeniz Technical University, Trabzon, TURKEY

4.

Department of Pharmacognosy, Faculty of Pharmacy, Karadeniz Technical University, Trabzon, TURKEY

5.

Department of Public Health, Faculty of Medicine, Karadeniz Technical University, Trabzon, TURKEY

6.

Surgical Application and Research Center, Faculty of Medicine, Karadeniz Technical University, Trabzon, TURKEY

Turkish J Nephrol 2023; 32: 140-147
DOI: 10.5152/turkjnephrol.2023.2227296
Read: 530 Downloads: 323 Published: 03 April 2023

Objective: The present study aimed to investigate the effect of Hypericum perforatum extract on amikacin-induced kidney disease in rats.

Methods: About 28 female Spraque-Dawley rats were separated into groups. The control, amikacin, amikacin+ H. perforatum extract, and H. perforatum extract groups were given intraperitoneally 1 mL of serum physiologic, amikacin (1.2 g/kg), H. perforatum extract (50 mg/kg) +amikacin (1.2 g/kg), and H. perforatum extract (50 mg/kg), respectively. On the third day, serum blood urea nitrogen, creatinine, malondialdehyde, superoxide dismutase, catalase, glutathione peroxidase, histopathology, and apoptosis were explored in kidney tissue.

Results: The serum blood urea nitrogen and creatinine levels were higher in H. perforatum extract-pretreated amikacin group than in the other groups. Kidney malondialdehyde levels which showed oxidative injury were lowest in the serum physiologic group and highest in amikacin and amikacin+H. perforatum extract groups. Anti-oxidative enzyme (superoxide dismutase, catalase, glutathione peroxidase) levels decreased more in amikacin and amikacin+H. perforatum extract groups than serum physiologic (control) and H. perforatum extract groups. Histopathological damage scores of the amikacin+H. perforatum extract group were higher than the other groups. Proximal tubular tissue necrosis was observed in all of the rats in amikacin and amikacin+H. perforatum extract groups. Amikacin and amikacin+H. perforatum extract groups demonstrated the highest apoptotic index percentage.

Conclusion: We found that H. perforatum extract increased kidney injury mostly through oxidative damage and induction of apoptosis.

Cite this article as: Kübra Kaynar R, Aliyazıcıoğlu R, Yenilmez E, et al. Hypericum perforatum extract increased necrosis in amikacin-induced kidney injury. Turk J Nephrol. 2023;32(2):140-147.

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