Turkish Journal of Nephrology
Original Article

Evaluation of Thiol/Disulfide Homeostasis and Other Oxidative Stress Markers in Patients Undergoing Hemodialysis


Department of Biochemistry, Demiroğlu Bilim University School of Medicine, İstanbul, Turkey


Department of Clinical Biochemistry, Yıldırım Beyazıt University School of Medicine, Ankara, Turkey


Eldivan Vocational School of Health Services, Çankırı Karatekin University, Çankırı, Turkey


Vocational School of Health Services, Demiroğlu Bilim University, İstanbul, Turkey

Turkish J Nephrol 2021; 30: 17-24
DOI: 10.5152/turkjnephrol.2021.4396
Read: 221 Downloads: 113 Published: 04 February 2021

Objective: To investigate the potential role of thiol/disulphide homeostasis as a novel biomarker of oxidative stress in patients with diabetes and undergoing hemodialysis (HD) and its correlation with other oxidative stress markers.

Materials and Methods: This study included 82 patients with end-stage renal disease undergoing HD for four hours, three times weekly for more than 24 months in the dialysis center. Of the 82 patients, 47 were non-diabetic and 35 were diabetic. Blood samples were collected from the patients before and after the HD sessions. The thiol/disulfide pair tests were performed and total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), ischemia-modified albumin (IMA) levels, albumin levels, ceruloplasmin, catalase activity (CAT), and myeloperoxidase (MPO) activity were determined in the serum. 

Results: The TAS values in all the patients, both diabetic and non-diabetic, decreased significantly after HD (p<0.001, p=0.003, and p<0.001; respectively). The TOS, albumin, native thiol (p=0.001, p=0.007, p=0.001, respectively), OSI, CAT, ceruloplasmin, IMA, MPO, and total thiol (p<0.001, p<0.001, p<0.001, p<0.001, p<0.001, and p<0.001, respectively) values increased significantly in all the patients after the HD session. The TOS, OSI, CAT, IMA, albumin, MPO, native thiol, and percentages of native/total thiol, ceruloplasmin, and total thiol values (p=0.002, p=0.002, p=0.002, p=0.001, p=0.008, p=0.001, p=0.001, p=0.003, p=0.023, and p<0.001; respectively) increased significantly in patients with diabetes after the HD session. 

Conclusion: In this study, we demonstrated the relationship between oxidative stress markers, which play a significant role in the pathogenesis of diabetes, and thiol/disulfide balance undergoing HD patients.

Cite this article as: Söğüt İ, Şenat A, Oğlakçı İlhan A, Sezen A, Erel Ö. Evaluation of Thiol/Disulfide Homeostasis and Other Oxidative Stress Markers in Patients Undergoing Hemodialysis. Turk J Nephrol 2021; 30(1): 17-24.

EISSN 2667-4440