Turkish Journal of Nephrology
Original Article

Effect of Epigallocatechin Gallate on Cisplatin-Induced Nephrotoxicity in Rats

1.

Department of Internal Medicine, Fethi Sekin City Hospital, Elazığ, Turkey

2.

Department of Nephrology, Tokat State Hospital, Tokat, Turkey

3.

Department of Nephrology, Fırat University School of Medicine, Elazığ, Turkey

4.

Department of Pathology, Fırat University School of Medicine, Elazığ, Turkey

5.

Department of Animal Nutrition, Fırat University Faculty of Veterinary Science, Elazığ, Turkey

Turkish J Nephrol 2021; 30: 262-268
DOI: 10.5152/turkjnephrol.2021.21027
Read: 259 Downloads: 91 Published: 13 October 2021

Objectives: Nephrotoxicity caused by CDDP is attributed to an increase in kidney oxidative stress, which restricts the therapeutic application of CDDP. EGCG is a very strong green tea-derived antioxidant. To investigate the effect of epigallocatechin gallate (EGCG) on cisplatin (CDDP)-induced lipid peroxidation and nephrotoxicity in rats.
Methods: Twenty-eight male Wistar rats (8 weeks, 200-215 g) were used in the research and were separated into 4 equal groups: (1) control rats, (2) EGCG (100 mg/kg, daily) control rats, (3) CDDP-injected rats, CDDP group (7 mg/kg, i.p., single dose), and (4) EGCG-treated (100 mg/kg, daily) plus CDDP-injected rats (EGCG + CDDP group).
Results: There was a substantial rise in malondialdehyde (MDA) levels in the CDDP-injected rats (P < .05). The EGCG prevented the rise of the MDA (P < .05). In CDDP-injected rats, renal Bax protein expression was substantially higher compared with control rats, and EGCG therapy significantly decreased Bax protein levels (P < .05). In EGCG + CDDPadministered rats, Bcl-2 protein expression was higher than in CDDP-injected rats (P < .05). In the EGCG + CDDP group, the expression of renal heat shock protein 60 (Hsp60) and heat shock protein 70 (HSP70) was significantly lower compared to CDDP-alone injected rats (P < .001). CDDP-induced renal histopathological changes were significantly improved with EGCG.
Conclusion: CDDP produces oxidative stress and renal damage. The EGCG that reduces oxidative stress and changes the expression of Bax and Bcl-2 proteins may potentially have a significant role in the prevention of CDDP-induced renal injury.
Cite this article as: T imurkaan M , D emir M , G ömleksiz M R, H anifi Ö zercan İ , Ş ahin K , D oğukan A . E ffect o f e pigallocatechin g allate o n cisplatin-induced nephrotoxicity in rats. Turk J Nephrol. 2021; 30(4): 262-268.

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